CHM 453 - Biochemistry Laboratory II

CHM 451 with a grade of C (2.0) or better; prior or concurrent election of CHM 452. (2)

Project-oriented laboratory in which students attempt to do a site-directed mutagenesis of a gene that codes for an enzyme, compare the physical and kinetic parameters of the mutated and wild type enzymes, and propose and test a hypothesis about how the mutation will affect the rate of enzymatic catalysis. Eight hours recitation/laboratory weekly. Graded ABCDE